%0 Journal Article %T Surface-Enhanced IR-Absorption Microscopy of Staphylococcus aureus Bacteria on Bactericidal Nanostructured Si Surfaces %+ P. N. Lebedev Physical Institute of the Russian Academy of Sciences [Moscow] (LPI RAS) %+ National Research University of Information Technologies, Mechanics and Optics [St. Petersburg] (ITMO) %+ N.F. Gamaleya Federal Research Centre N.F. Gamaleya Federal Research Centre of Epidemiology and Microbiology (Gamaleya) %+ FSBI TISNCM, 108840 Moscow, Russia (TISNCM) %+ Gamaleya Research Center %+ B. I. Stepanov Institute of Physics [Minsk] %+ Laboratoire Hubert Curien (LHC) %A Kudryashov, Sergey, I %A Nastulyavichus, Alena, A %A Tolordava, Eteri, R %A Kirichenko, Alexey, N %A Saraeva, Irina, N %A Rudenko, Andrey, A %A Romanova, Yulia, M %A Panarin, Andrey Yu. %A Ionin, Andrey, A %A Itina, Tatiana %< avec comité de lecture %@ 1420-3049 %J Molecules %I MDPI %V 24 %N 24 %P 4488 %8 2019-12 %D 2019 %R 10.3390/molecules24244488 %K Si nanostructures %K staphylococcus aureus bacterial biofilm %K bactericidal effect %K surface-enhanced IR microscopy %K chemical enhancement %Z Engineering Sciences [physics]/Chemical and Process Engineering %Z Engineering Sciences [physics] %Z Physics [physics]Journal articles %X Surface-enhanced IR absorption (SEIRA) microscopy was used to reveal main chemical and physical interactions between Staphylococcus aureus bacteria and different laser-nanostructured bactericidal Si surfaces via simultaneous chemical enhancement of the corresponding IR-absorption in the intact functional chemical groups. A cleaner, less passivated surface of Si nanoripples, laser-patterned in water, exhibits much stronger enhancement of SEIRA signals compared to the bare Si wafer, the surface coating of oxidized Si nanoparticles and oxidized/carbonized Si (nano) ripples, laser-patterned in air and water. Additional very strong bands emerge in the SEIRA spectra on the clean Si nanoripples, indicating the potential chemical modifications in the bacterial membrane and nucleic acids during the bactericidal effect. %G English %2 https://ujm.hal.science/ujm-02978946/document %2 https://ujm.hal.science/ujm-02978946/file/molecules-24-04488.pdf %L ujm-02978946 %U https://ujm.hal.science/ujm-02978946 %~ UNIV-ST-ETIENNE %~ IOGS %~ CNRS %~ PARISTECH %~ UDL