%0 Journal Article %T Automatic numerical focus plane estimation in digital holographic microscopy using calibration beads %+ Laboratoire Hubert Curien (LHC) %+ Centre Christophe Mérieux %+ BIOASTER Microbiology Technology Institute [Lyon] %+ Laboratoire de Mecanique des Fluides et d'Acoustique (LMFA) %+ Centre National de la Recherche Scientifique (CNRS) %A Brault, Dylan %A Fournier, Corinne %A Olivier, Thomas %A Faure, Nicolas %A Dixneuf, Sophie %A Thibon, Louis %A Mees, Loic %A Denis, Loïc %< avec comité de lecture %@ 1559-128X %J Applied optics %I Optical Society of America %V 61 %N 5 %P B345 %8 2022-01-28 %D 2022 %R 10.1364/AO.444996 %K focus plane %K inverse problems approach %K autofocus %K digital holographic microscopy %K calibration beads %Z Computer Science [cs]/Image Processing [eess.IV] %Z Engineering Sciences [physics]/Signal and Image processingJournal articles %X We present a new method to achieve autofocus in digital holographic microscopy. The method is based on inserting calibrated objects into a sample placed on a slide. Reconstructing a hologram using the inverse problems approach makes it possible to precisely locate and measure the inserted objects and thereby derive the slide plane location. Numerical focusing can then be performed in a plane at any chosen distance from the slide plane of the sample in a reproducible manner and independently of the diversity of the objects in the sample. %G English %2 https://ujm.hal.science/ujm-03571296/document %2 https://ujm.hal.science/ujm-03571296/file/2022_AO_Brault_Fournier_Autofocus_DH.pdf %L ujm-03571296 %U https://ujm.hal.science/ujm-03571296 %~ UNIV-ST-ETIENNE %~ IOGS %~ CNRS %~ UNIV-LYON1 %~ INSA-LYON %~ EC-LYON %~ PARISTECH %~ LMFA %~ PRIMES %~ PRIMES_WP4 %~ INSA-GROUPE %~ UDL %~ UNIV-LYON %~ ANR %~ EC_LYON_STRICT