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Journal Articles Talanta Year : 2007

Characterization of different diamond-like carbon electrodes for biosensor design

Abstract

Diamond-like carbon (DLC) films are gaining big interest in electrochemistry research area. DLC electrodes made with different ratio of sp3/sp2 carbon hybridization or doped with different percentages of nickel were characterized electrochemically by cyclic voltammetry and by amperometric measurements towards hydrogen peroxide. SiCAr1 and SiCNi5% were chosen as sensitive transducers for the elaboration of amperometric glucose biosensors. Immobilization of glucose oxidase was carried out by cross-linking with glutaraldehyde. Measurements were made at a fixed potential + 1.0V in 40mM phosphate buffer pH 7.4. SiCAr1 seems to be more sensitive for glucose, 0.6875 µA/mM, than SiCNi5%, 0.3654 µA/mM. Detections limits were 20 µM and 30µM, respectively. Apparent Michaelis-Menten constants were found around 3 mM. Forty-eight percent and 79% of the original response for 0.5mM glucose remained after 10 days for both biosensors, respectively.
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Dates and versions

ujm-00140042 , version 1 (04-04-2007)

Identifiers

  • HAL Id : ujm-00140042 , version 1

Cite

R. Maalouf, H. Chebib, Y. Saikali, O. Vittori, M. Sigaud, et al.. Characterization of different diamond-like carbon electrodes for biosensor design. Talanta, 2007, 72, pp.310-314. ⟨ujm-00140042⟩
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