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Journal Articles Biomedical optics express Year : 2018

Wavefront division digital holographic microscopy

Nimit Patel
  • Function : Author
Vismay Trivedi
  • Function : Author
Swapnil Mahajan
  • Function : Author
  • PersonId : 929261
Vani Chhaniwal
  • Function : Author
Corinne Fournier
Seonoh Lee
  • Function : Author
Arun Anand
  • Function : Author

Abstract

Digital holographic microscopy is the state of the art quantitative phase imaging of micro-objects including living cells. It is an ideal tool to image and quantify cell thickness profiles with nanometer thickness resolution. Digital holographic techniques usually are implemented using a two-beam setup that may be bulky and may not be field portable. Self-referencing techniques provide compact geometry but suffer from a reduction of the field of view. Here, we discuss the development of a wavefront division digital holographic microscope providing the full field of view with a compact system. The proposed approach uses a wavefront division module consisting of two lenses. The developed microscope is tested experimentally by measuring the physical and mechanical properties of red blood cells.

Dates and versions

ujm-01903211 , version 1 (24-10-2018)

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Nimit Patel, Vismay Trivedi, Swapnil Mahajan, Vani Chhaniwal, Corinne Fournier, et al.. Wavefront division digital holographic microscopy. Biomedical optics express, 2018, 9 (6), ⟨10.1364/boe.9.002779⟩. ⟨ujm-01903211⟩
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