Characterization of different DLC and DLN electrodes for biosensor design - Université Jean-Monnet-Saint-Étienne Access content directly
Journal Articles Journal of Advanced Science Year : 2006

Characterization of different DLC and DLN electrodes for biosensor design

Abstract

Diamond-Like Carbon and Carbon-Like Nanocomposite electrodes, novel materials in the field of biosensors, made with different ratio of sp3/sp2 carbon hybridization or doped with elements such as Ni, Si and W, were characterized electrochemically by cyclic voltammetry and by amperometric measurements towards hydrogen peroxide. SiCAr1 and SiCNi5% were chosen as sensitive transducers for elaboration of amperometric glucose biosensors. Immobilization of glucose oxidase was carried out by cross-linking with glutareldehyde. Measurements were made at a fixed potential + 1.0 V in 40 mM phosphate buffer pH 7.4. SiCAr1 seems to be more sensitive for glucose (0.6875 µA/mM) then SiCNi5% (0.3654 µA/mM). Detections limits were respectively 20 µM and 30 µM. Michaelis-Menten constants for the two electrodes were found around 3 mM. 48% and 79% of the original response for 0.5 mM glucose remained respectively for both electrodes after 10 days.
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Dates and versions

ujm-00109718 , version 1 (26-10-2006)

Identifiers

  • HAL Id : ujm-00109718 , version 1

Cite

R. Maalouf, N. Jaffrezic-Renault, O. Vittori, M. Sigaud, Y. Saikali, et al.. Characterization of different DLC and DLN electrodes for biosensor design. Journal of Advanced Science, 2006, 18, 18, pp.31-36. ⟨ujm-00109718⟩
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